The layoffs are the major portion of a US$400 million cost-cutting plan Yahoo is implementing that also includes other measures for achieving what the company called "structural efficiencies."
Revenue for the third quarter, which ended Sept. 30, increased only 1 percent to $1.768 billion compared with the same quarter in 2007.
Subtracting the commissions Yahoo pays to its advertising partners, revenue was $1.325 billion, up 3 percent.
Chief Financial Officer Blake Jorgensen said in Tuesday's statement that the company's revenue in the quarter was disappointing, and blamed the troubles on the tough economy and lower demand for advertising.
Net income came in at $54 million, or $0.04 per share, down from net income of $151 million, or $0.11, in 2007's third quarter.