The news comes it plans to create an additional 2,000 permanent roles in the UK up until the end of 2014.
The new London centre, located in Glasshouse Yard near Barbican, will focus on digital media projects that are set to include the creation of interactive digital services for TVs, game consoles, smartphones, PCs, as well as new APIs.
Boris Johnson, Mayor of London, hailed the announcement and opening as another success story for the capital attracting investment from international technology firms.
"It's fantastic to welcome Amazon's new tech hub to London, giving the capital yet another vote of confidence from the world's leading innovators," said Johnson.
"Boosting London's tech and media workforce is key to driving the capital's economy and helping to create jobs and growth."